Ratio-Metric Fluorescence/Colorimetric and Smartphone-Assisted Visualization for the Detection of Dopamine Based on Cu-MOF with Catecholase-Like Activity

As the most abundant catecholamine neurotransmitter in brain, dopamine plays an important role normal physiological process, and its level urine also changes during human pathological processes. In clinic, detection of is a potential marker for diagnosis treatment endocrine-related diseases. this work, copper metal organic framework with catecholase-like activity was prepared via precipitation Cu2+ imidazole, simulating N-Cu coordination environment active site catecholase. Cu-MOF (the copper–metal framework) can catalyze oxidation DA (dopamine) to dopaquinone using O2 air. The product further react 1,3-dihydroxynaphthalene produce fluorophore product. Based on above reaction, multimodal sensing platform three signal outputs, including ratio-metric fluorescence, absorbance digital information extracted from smartphone images simple sensitive determination DA, proposed, limits 0.0679, 0.3206, 0.3718 μM, respectively. This able detect body fluid self-correcting way, as demonstrated by successful samples, samples high interference.